Journal: Frontiers in Plant Science
Article Title: DESI-MSI applications for direct (spatial) biomolecular analysis of South African natural medicinal products
doi: 10.3389/fpls.2026.1802351
Figure Lengend Snippet: High-performance thin-layer chromatography (HPTLC) analysis of South African propolis extract. The representative plate was visualized under (A) UV 366 nm, (B) white light reflectance, and (C) a bioautogram showing the antibacterial activity of selected bands against S. pneumoniae . (D) a DESI-MSI overlay of all bands present within the antibacterial region. (E) 1 – (E) 9 DESI–MS images of selected chromatographic bands, identified as: (E) 1 , Pinobanksin; (E) 2 , Chrysin; (E) 3 , Galangin; (E) 4 , Pinobanksin acetate; (E) 5 , Pinobanksin propionate; (E) 6 , Pinocembrin; (E) 7 , Pinobanksin butyrate; (E) 8 , Pinobanksin pentanoate; and E 9 , Pinobanksin hexanoate. Ion intensity signals were normalized to the total ion current and displayed as a heat map. Scale bar = 11.55 mm.
Article Snippet: Haemophilus Test Medium (HTM), HTM Supplement SR0158, Streptococcus pneumoniae (ATCC 6841) was sourced from University of Witwatersrand (Johannesburg, South Africa), super pure-grade methanol was obtained from Romil (Johannesburg, South Africa).
Techniques: High Performance Thin Layer Chromatography, Activity Assay